Protein
Western Blot Protocol
Posted March 5th, 2008 by Stephen
Discipline: Protein
Keywords: protein, western, western gel, westerns, loading buffer, running buffer
Keywords: protein, western, western gel, westerns, loading buffer, running buffer
Materials:
How to choose a method for identifying DNA-protein interaction
Posted January 25th, 2008 by lizzer
Discipline: Molecular biology, Protein
Keywords: yeast one hybrid, oligonulcotide screening, method, in vitro expression cloning, hybrid screening, gel shift, fractionation, expression cloning, DNA-protein interaction, compare and contrast, chromatography, biochemical purification
Keywords: yeast one hybrid, oligonulcotide screening, method, in vitro expression cloning, hybrid screening, gel shift, fractionation, expression cloning, DNA-protein interaction, compare and contrast, chromatography, biochemical purification
There are many techniques and strategies that you can use to identify a DNA-protein interaction (when the DNA fragment is known). Here are some pros and cons for each, to help you choose which is best for your situation.
1. Gel shift assay
How:
perform gel shift under stringent binding conditions (high [salt], low temperature)
Pros:
Cons:
2. Biochemical purification of binding proteins
