Plant biology
Cell wall isolation
Posted January 28th, 2008 by JennyT
Discipline: Plant biology
Keywords: lignin, cellulose, cell wall, extraction, plant, biomass, chemistry, biochemistry, carbon
Keywords: lignin, cellulose, cell wall, extraction, plant, biomass, chemistry, biochemistry, carbon
Lignocellulose Extraction (modified from Crawford and Crawford, 1976)
1. Grind plant material with liquid nitrogen (LN), a mortar and pestle twice until the
material is the consistency of flour.
2. Place 25.0-g plant material with 250-mL cold DI water in a 1-L round bottom flask. Set up apparatus (see attached diagram) so flask is situated in tap water in a crystallizing dish, such that the water-line inside the flask is almost (but not completely) submerged in the water inside the crystallizing dish. Stir at approx. 600 rpm.*
1. Grind plant material with liquid nitrogen (LN), a mortar and pestle twice until the
material is the consistency of flour.
2. Place 25.0-g plant material with 250-mL cold DI water in a 1-L round bottom flask. Set up apparatus (see attached diagram) so flask is situated in tap water in a crystallizing dish, such that the water-line inside the flask is almost (but not completely) submerged in the water inside the crystallizing dish. Stir at approx. 600 rpm.*
